Figure 5.

(a) COUP-TFII promotes the adipogenic differentiation of mesenchymal cells. COUP-TFII siRNA-treated BMSC cells and controlled BMSC cells underwent adipocyte differentiation, and cells were stained with oil red O and photographed on day 14. Decreasing adipogenesis was noted after COUP-TFII knockdown (magnification, 200x). (b and c) Impaired adipogenesis in COUP-TFII-depleted cells was confirmed by western blotting, with decreased expression of PPARγ and C/EBP-α. (AM: adipogenic medium; KD: knockdown; Data are presented as mean ± SD; t test, ^∗∗P < 0.01, ^∗∗∗P < 0.001.)