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(a) Alizarin red staining of BMSCs that underwent osteogenesis for 28 days. When cultured in osteogenic medium, COUP-TFII knockdown BMSCs showed increased osteogenic differentiation compared with control BMSCs (magnification, 200x). (b and c) Increased osteogenesis in COUP-TFII-depleted cells was confirmed by western blotting with increased expression of Runx2. (OM: osteogenic medium; KD: knockdown; data are presented as the means ± SD; t test, ∗∗P < 0.01.)
