Fig. 3.

Spontaneous gonadal teratomas occur in Dazl-deficient mice, and arise from mitotic cells. (A) Appearance of unilateral gonadal teratomas (arrows) in 129S4.Dazl-deficient mice. (B) Incidence of gonadal teratomas in mice. Males were dissected by 4 mo (adult male) or at 4 wk of age (juvenile male), and adult females were dissected at 2 mo of age; n = number of animals examined, ***P value ≤ 0.0001 using Fisher’s exact test. (C) Representative histology of teratomas from testis (Upper) and ovary (Lower) stained with periodic acid-Schiff reagent (PAS). (Scale bar, 100 µm.) (D) Representative Sanger sequencing at 4 SNP loci from 129S2 and 129S4 mice (Upper), and from 129SF1 host and teratoma (Lower). Teratomas are heterozygous at each SNP locus.