Fig. 5.
Microglial vascular repair is blocked by a fibrinogen-derived inhibitory peptide. (A and C) Frozen sections of lumbar spinal cord taken from mice maintained under hypoxic conditions for 7 d that received daily injections of either the fibrinogen-derived inhibitory peptide γ377-395 or a scrambled peptide, were stained for CD31 (Alexa Fluor 488) and fibrinogen (Cy-3) in A or CD31 (Alexa Fluor 488), fibrinogen (Cy-5, blue), and Mac-1 (Cy-3) in C. (B) Quantification of the number of leaky vessels/FOV. Results are expressed as the mean ± SEM (n = 6 mice per group). *P < 0.05. One-way ANOVA followed by Tukey’s multiple comparison test. Note that mice treated with the inhibitory peptide γ377-395 showed a greater number of leaky blood vessels and the peptide prevented the migration and accumulation of activated microglia around leaky blood vessels (C). (Scale bars, A, 50 μm; C, 25 μm.)