Fig. 8.

Nitrogen-15 chemical shift difference correlations involving wt* and Δ10 ngMinE constructs. (A) Correlation of the ¹⁵N chemical shift differences for residues 5 to 12 (encompassing helix α1 in the wt* construct) between folded and random coil wt* and between the A (ground) and B (excited) states (Left) and between the B and C excited states (Right) of wt*. The random coil shifts for residues 5 to 12 were calculated from the sequence using the algorithm of ref. 40. (B) Correlation of the ¹⁵N chemical shift differences for the loop preceding strand β1 (residues 14 to 19; solid red circles), strand β1 (open red circles), and strand β3 (open blue circles) between wt* and Δ10 in the wt*/Δ10 heterodimer and between the A (ground) and B (excited) states of wt*. (C) Correlation of the ¹⁵N chemical shift differences between the A (ground) and B (excited) states of Δ10 and the B and C excited states of wt*.