Fig. 9.

Correlation of ¹⁵N chemical shift changes (Δω) between major (ground) and sparsely populated (excited) states for wt* and Δ10 determined from ¹⁵N CPMG relaxation dispersion and the ¹⁵N chemical shift differences between 6-stranded and 4-stranded ground states of ngMinE. (A) Correlation between Δω_AC values for the wt* A–C transition and the ¹⁵N chemical shift differences between wt* (6-stranded) and I24N (4-stranded) constructs. (B) Correlation between Δω_AB values for the Δ10 A–B transition and the ¹⁵N chemical shift differences between wt* (6-stranded) and Δ10/I24N (4-stranded) constructs. (Top) The correlations for strand β1 (solid circles) as well as residues in loops preceding and following strand β1 (open circles); the arrow for residue 24 serves to indicate the chemical shift difference between wt* and predicted random coil values since this residue is mutated from Ile to Asn in the 4-stranded constructs. (Bottom) The correlations for strands β2 (open circles) and β3 (solid circles). The lines serve purely to guide the eye.