Fig. 4.

[³H]NMS binding dissociation kinetic studies of chimeric swaps between the ECLs of the M₂ and M₅ mAChRs. (A) Cartoons for the M₂ and M₅ ECL chimeras used in this study. (B) [³H]NMS reassociation was prevented by the addition of 10 µM atropine, and radioligand dissociation was monitored in the absence (Vehicle) or presence of 10 µM ML375 or 10 µM 4P-C₇/3-phth. Data points represent the mean ± SEM of 3 or more independent experiments performed in duplicate. (C) Comparison of the dissociation half-lives for [³H]NMS, showing both individual values and the mean ± SEM (log-scale). Full quantitative parameters derived from this experiment are listed in SI Appendix, Table S5, including a statistical analysis.