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. 2019 Dec 3;116(51):25991–26000. doi: 10.1073/pnas.1902483116

Fig. 4.

Fig. 4.

Superoxide dismutase activity of hSod1 homo- and heteromers. (A) Representative immunoblot confirming expression of VN–Sod1 and Sod1–VC fragments in yeast cells, both before and after aging conditions. (B) Superoxide dismutase activity was performed in exponential-phase sod1Δ cells expressing the hSod1, before and after chronological aging. Values are means ± SD of at least 3 independent experiments and represent the ratio of hSod1 activity between aged and nonaged yeast cells. * represents statistically different results between mutant hSod1 with the WT hSod1 variant (mutSod1 vs. WTSod1). Colored bars represent mutant homodimers, and hatched colored bars represent mutant heterodimers. (C) Sod1 activity of yeast sod1Δ cells expressing only the hSod1 VN or only presenting the VC fragment, in WT or A4V hSod1. Sod activity was performed in exponential-phase cells before chronological aging.