Fig. 5.

Expression of WT–mutant heteromers decreases cellular viability after 24 h aging. Yeast cell lacking endogenous Sod1 (sod1Δ) as well as carrying hSod1 (WT and mutants) were harvested at exponential phase and aged in water (nonproliferating condition) at 37 °C for 24 h. Cellular viability was carried out by counting plated cells on dropout 2% and expressed as the percentage of viable cells. Hatched and nonhatched colored bars represent mutant heterodimers and homodimers, respectively. The results represent the mean ± SD of at least 3 independent experiments. * represents statistically different results between sod1Δ or hSod1 mutants with the WT hSod1 variant (sod1Δ or mutants Sod1 vs. WT Sod1). ** statistically different results between recombinant yeast cells expressing hSod1 with the respective nonrecombinant strain, sod1Δ (WT or mutants hSod1 vs. sod1Δ).