Fig. 7.

Chronological aging induces the formation of mutant hSod1 cytoplasmic inclusions. (A) Representative pictures showing the localization of BiFC–hSod1 mutants in yeast cells imaged by fluorescence microscopy, both before and after aging. Arrows indicate some hSod1 inclusions. (Scale bar: 10 µm.) (B) Quantification of the number of inclusions per cell. At least 50 cells were counted per condition and organized in 3 different categories which represent: the percentage of cells without any inclusion (blue bar), with 5 or less inclusions (purple bar), and yeast cells with more than 5 inclusions (yellow bars). One-way ANOVA, with Bonferroni correction, was used for statistical analysis with significance level of ***P < 0.001 (WT vs. mutant cells). **P < 0.01 shows the significance level between WT and mutant cells. Data were combined from at least 3 independent experiments.