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. 2019 Nov 26;116(51):25923–25931. doi: 10.1073/pnas.1910883116

Fig. 2.

Fig. 2.

MAIT cell activation by fixed GAS is dependent on IL-12 and IL-18, but not on MR1. MAIT cells were incubated with monocytes stimulated with fixed bacteria for 24 h, and frequency of activated cells was assessed by flow cytometry. (A–C) Stimulation with fixed E. coli or GAS 2006 in the presence of anti-MR1 antibody or IgG2A isotype control. (A and C) IFNγ+CD69+ MAIT cells in (A) 1 representative donor and (C) mean ± SD of 4 donors. (B) Vα7.2 expression presented as geoMFI. Mean ± SD of 4 donors. (D) Frequencies of IFNγ+ CD69+ or TNF+ CD69+ MAIT cells after stimulation with fixed GAS 2006 in the presence of anti-IL-12 and/or anti-IL-18 antibody or IgG1 isotype control. Mean ± SD of 3 donors. (B–D) One-way ANOVA was used to detect significant differences between paired samples. **P < 0.01; *P < 0.05.