Fig. 4.

Hhex expression is increased under type I inflammatory conditions in vivo. DTH reaction was induced by double topical applications of oxazolone (day 0, sensitization and day 7, challenge) on the mice back skin. The mice were killed at 2 d after challenge and analyzed for disease phenotypes. (A) H&E staining of back skin section. (B) Total CD4 T cell numbers in draining lymph nodes (dLNs) of untreated control and oxazolone-treated DTH groups were counted. (C) Populations of cytokine-expressing CD4 T cells in dLNs. (D–F) CD4⁺ CD25⁺ Treg cells were isolated from dLNs of control and DTH mice. Total RNA was extracted and expression of Hhex, Foxp3, and Ifng was measured by qRT-PCR. All error bars represent the SD, and P values were calculated using Student’s t tests. *P < 0.05, **P < 0.01.