Fig. 3.

Genomic organization, expression, and function of genes involved in digestion of polysaccharides. (A) Syntenic loci of GHs, transporter genes, and transcriptional regulators in B. asteroides and Bifidobacterium bohemicum strains. Homologous genes are connected by gray bars, and the GH family number is shown for respective genes. (B) Gene expression profiles of GHs in response to different hemicellulose substrates. Error bars represent SDs of 3 biological replicates. (C) Results of partial least squares discriminant analysis based on 687 metabolites detected from guts of W8111-colonized or W8103-colonized bees fed on sucrose supplemented with arabinan, galactan, or xyloglucan. Six biological replicates for each treatment. (D–F) Volcano plots of differentially abundant metabolites identified in guts of monoassociated bees fed on different polysaccharides.