Skip to main content
. 2019 Dec 3;116(51):25850–25859. doi: 10.1073/pnas.1902836116

Fig. 6.

Fig. 6.

B cell responses to a BCR-specific T-independent type-2 antigen is enhanced by Id-specific T cells. (A) Experimental setup (n = 5 Id-sp. T/DNP-FICOLL; n = 5 Id-sp. T/NIP-FICOLL; n = 3 DNP-FICOLL). Spleens were analyzed by IHC (B and C) or by FACS (D and E). (B) Representative cryosection from a recipient mouse spleen immunostained using anti-λ BCR L chain Alexa Fluor 488 (green), peanut agglutinin Alexa Fluor 594 (red), and rat anti-mouse reticular fiber Ab (ER-TR7, blue). (C) GCs quantified from immunostained spleen sections. (D) Germinal center B cells. GL7+ cells with λ L chains were gated among IgD− B cells (SI Appendix, Fig. S17). (E) Plasma cells (CD138+) among splenocytes. (F) Serum concentrations of anti-Id Ig-reactive IgM, IgG, and DNP-reactive IgG on day 10. (G) CD40L blockade by repetitive injection of anti-CD40L mAb or isotype-matched control. Effects on serum levels of Id+ IgM (day 5) and IgG1 (day 7). A detailed description and analysis is given in SI Appendix, Fig. S18. Statistical comparisons: Tukey’s multiple comparisons tests (C–E), Dunn's multiple comparisons test (F and G). *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.005.