Figure 7. Androgen-dependent prostate cancer cell lines are more sensitive to natamycin treatment.

A. LNCaP cells were plated in 10 % FBS supplemented medium at 10⁴ cells per well onto E-plates and treated with vehicle, 0.1 μM, 1 μM, or 10 μM of natamycin. B. LNCaP cells were plated in medium supplemented with 10% CSS at 10⁴ cells/well and treated as in A. C. PNT1A cells were plated in medium supplemented with 10% CSS at 5x10⁴ cells per well and treated as in A. D. LNCaP cells were plated in medium supplemented with 10% CSS or 10% FBS and cells were allowed to attach. Next day, cells were treated with vehicle, 0.1 μM, 1 μM, 2.5 μM, 5 μM, or 10 μM of natamycin. Forty eight hours later cellular viability was compared using MTT assay. E. LNCaP cells were grown in complete medium and treated for 48 h with 10 μM of indicated compounds. Protein was extracted and AR, γ-H2AX, and tubulin levels compared by Western blotting. Impedance in A-C was measured every 30 min and values were normalized to those at the time of treatment. (*signifies a difference between vehicle and treatment with ** p≤ 0.01, ****p≤ 0.0001).