Figure 1.

lncRNA MALAT1 Is Upregulated and BRCA1 Is Downregulated in Skeletal Muscle Tissues of Septic Mice

(A) The degree of skeletal muscle tissue injury in septic mice observed by H&E staining (×400). (B) The number of neutrophils in peripheral blood in septic mice measured by flow cytometry. (C) The serum levels of IL-6, TNF-α, IL-8, IL-10, TGF-β, and IL-13 measured by ELISA. (D) The apoptosis of skeletal muscle cells measured by TUNEL staining (×400). (E) The expression of MALAT1 and BRCA1 in skeletal muscle tissues determined by qRT-PCR. (F) The expression of BRCA1 normalized to GAPDH in skeletal muscle tissues determined by western blot analysis. *p < 0.05 versus the blank group or the sham group. The statistical values were measurement data, expressed as mean ± SD, and were analyzed with one-way ANOVA, followed by Tukey’s post hoc test. N = 10. The experiment was repeated three times independently.