Figure 2.

lncRNA MALAT1 Affects the Expression of BRCA1 in Human Skeletal Muscle Cells (HSMKMC 3500) of Sepsis

(A) The expression of lncRNA MALAT1 and BRCA1 in normal skeletal muscle cells and septic skeletal muscle cells determined by qRT-PCR (the control group represents normal skeletal muscle cells; the sepsis group represents the LPS-induced skeletal muscle cell model of sepsis). (B) The silence efficiency of lncRNA MALAT1 in the skeletal muscle cell model of sepsis determined by qRT-PCR (blank, skeletal muscle cell model of sepsis; sh-NC, skeletal muscle cells in sepsis transfected with sh-NC; sh-MALAT1, skeletal muscle cells in sepsis transfected with sh-MALAT1). (C) The apoptosis of skeletal muscle cells measured by flow cytometry. (D) The expression of BRCA1 and BRCA1 in skeletal muscle cells of sepsis determined by qRT-PCR and western blot analysis. (E) The expression of BRCA1 normalized to GAPDH in the nucleus and cytoplasm determined by western blot analysis. (F) The subcellular localization of BRCA1 in skeletal muscle cells of sepsis detected by an immunofluorescence assay (×400). *p < 0.05 versus the control, blank, or sh-NC group. The statistical values were measurement data, expressed as mean ± SD. The data between two groups were analyzed with an independent samples t test, and the data among multiple groups were analyzed using one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated three times independently.