Figure 3.

lncRNA MALAT1 Regulates Expression of BRCA1 via Recruiting EZH2 in Skeletal Muscle Cells of Sepsis

(A) The predicted subcellular location of lncRNA MALAT1 by the lncATLAS website. (B) The co-location of lncRNA MALAT1 and EZH2 in skeletal muscle cells of sepsis determined by RNA-FISH (original magnification, ×400). (C) The binding of lncRNA MALAT1 and EZH2 detected by RIP assay. *p < 0.05 versus the IgG group. (D) The binding of lncRNA MALAT1 and EZH2 confirmed by an RNA pull-down assay. (E) The binding of truncated lncRNA MALAT1 with EZH2 by RIP assay. *p < 0.05 versus the GST group. (F) The expression of BRCA1 normalized to GAPDH determined by western blot analysis. (G) The expression of BRCA1 in the nucleus and cytoplasm normalized to LamininB1 and GAPDH, respectively, determined by western blot analysis. (H) The apoptosis of cells measured by flow cytometry. *p < 0.05 versus the sh-NC group; ^#p < 0.05 versus the oe-NC group; ^&p < 0.05 versus the oe-EZH2 group. The statistical values were measurement data and are expressed as mean ± SD. The data between two groups were analyzed with an independent samples t test, and the data among multiple groups were analyzed using one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated three times independently.