Fig. 5.

Specific forms of ionic liquids induce tetra-methylpyrazine production in C. glutamicum.

The production profiles of the C. glutamicum (JBEI-19571) against three types (imidazolium, cholinium, and protic form) of ionic liquids were examined in CGXII media with 4% d-glucose in a 24 well plate format. ([C₂C₁im]⁺); cholinium ([Ch]⁺); ethanolamine acetate [ETA][OAc]; and diethanolamine acetate [DEOA][OAc] A. Produced titers of tetra-methylpyrazine are shown at 48 ​h post induction under 75 ​mM (A) or 150 ​mM (B) [C₂C₁im][OAc], [Ch][OAc], [ETA][OAc], and [DEOA][OAc]. The control experiment was performed without IL supplementation (No ILs). Data shown are an average of biological triplicates, and the error bars represent standard error.