Fig. 6.

Fed-batch mode production of TMP in engineered C. glutamicum.

C. glutamicum (JBEI-19571) was initially cultivated in batch mode using CGXII minimal media including 8% starting d-glucose and 50 ​mM [Ch][Lys] in a 2L Sartorius Bioreactor. Subsequently, fed-batch mode was initiated when the initial d-glucose concentration decreased below 1%. A pulse mode feeding strategy was utilized to raise the d-glucose concentration above 1%. Final products and organic acid concentrations were quantified and are indicated as labeled in the legend above.