Table 1.
Strains and plasmids used in this study.
| Strain | Description | Selection | Reference |
|---|---|---|---|
| JBEI-7936 | Corynebacterium glutamicum ATCC 13032/NHRI 1.1.2, biotin auxotroph | NxR | Sasaki et al. (2019) |
| JBEI-19571 | JBEI-7936 harboring p/JBEI-19559 | KanR | Sasaki et al. (2019) |
| JBEI-19652 | JBEI-7936 harboring p/JBEI-19628 | KanR | This study |
| JBEI-19658 | JBEI-7936 harboring p/JBEI-19634 | KanR | This study |
| JBEI-19566 | JBEI-7936 ΔpoxB ΔldhA | SucR, KanS | Sasaki et al. (2019) |
| E. coli DH1 | F−λ–endA1 recA1 relA1 gyrA96 thi-1 glnV44 hsdR17(rK–mK–) | Meselson and Yuan (1968) | |
|
E. coli DH10β |
F− endA1 deoR+ recA1 galE15 galK16 nupG rpsL Δ(lac)X74 ϕ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) StrR λ– |
Invitrogen |
|
| Plasmid |
Description |
Selection |
Reference |
| JBEI-2600 | pEC-XK99E, E. coli-C. glutamicum shuttle expression vectors based on the medium-copy number plasmid including pGA1, KanR, oriV, Ptrc | KanR | Kirchner and Tauch (2003) |
| JBEI-19559 | pEC-XK99E-AK-IP-bypass | KanR | Sasaki et al. (2019) |
| JBEI-19628 | pTE221 pEC-XK99E-AK-IP-bypass-S. aureus mvaK1, mvaS (substitution) | KanR | This study |
| JBEI-19634 | pTE222 pEC-XK99E-AK-IP-bypass-C. kroppenstedtii mvaK1, mvaA (substitution) | KanR | This study |