Figure 3.

Characterization of MGP2-full. (A) CD spectra of 1.7μM MGP2-full at pHs ranging from 4 to 7, in10 mM sodium acetate (pH 4 and 5), or 20 mM sodium phosphate (pH 6 and 7) containing 0.5 % DHPC:DMPC (3:1) bicelles. B) Density-matched SV- AUC of MGP2-full at 11 μM at pH 4 and 7 in 10 mM sodium acetate, 150mM NaCl containing 0.5 % DPC. All experiments were fit to a single component model with the baseline offset floated (fit shown as solid line). (C) Density-matched SV-AUC of MGP2-full at 11 μM at pH 4 in 10 mM sodium acetate, 150mM NaCl containing DHPC:DMPC (3:1) bicelles. (D) Similar SV-AUC studies and analysis of MGP2-full at multiple concentrations (11 μM, 30 μM and 110 μM) in at pH 4 10 mM sodium acetate, 150 mM NaCl containing DHPC:DMPC (3:1) bicelles. The insert plots the protein concentration dependence of the sedimentation coefficient. (E) Liposomal release assay of MGP2-full in 10 mM sodium acetate, 100mM NaCl, Zn²⁺ at pH 4 or 10 mM sodium phosphate, 100mM NaCl, Zn²⁺ at pH 7.