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. 2019 Nov 12;19:84–96. doi: 10.1016/j.omtn.2019.10.034

Figure 5.

Figure 5

GAS5 Downregulation Prevents Atherosclerosis Progression in ApoE−/− Mice

(A) Transfection efficiency of GAS5 in each group detected by qRT-PCR and (B) transfection efficiency of sh-ABCA1 in each group detected by RT-qPCR. (C) Oil red O staining analysis of aortic plaque formation in C57BL/6J and ApoE−/−mice. (D) H&E staining analysis of aortic plaque formation in C57BL/6J and ApoE−/− mice (×400). (E) Serum cholesterol efflux in ApoE−/− mice with atherosclerosis after oe-GAS5, sh-GAS5, or sh-ABCA1 treatment. (F) Oil red O staining analysis of aortic plaque formation in ApoE−/− mice with atherosclerosis after oe-GAS5, sh-GAS5, or sh-ABCA1 treatment. (G) H&E staining analysis of aortic plaque formation in ApoE−/− mice with atherosclerosis after oe-GAS5, sh-GAS5, or sh-ABCA1 treatment (×400). *p < 0.05 versus the pLV-EGFP-N group; #p < 0.05 versus the sh-NC group; &p < 0.05 versus sh-ABCA1 group. The experiment was repeated three times. Comparison between two groups was analyzed using t test and comparison among multiple groups was analyzed using one-way ANOVA.