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. 2019 Nov 21;17:49–57. doi: 10.1016/j.omtm.2019.11.008

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Transport Activity Evaluation of PEPT1/SLC15A1

(A) TEER values of the WT iPSC-IEC and PEPT1-KO iPSC-IEC monolayers were measured by Millicell-ERS2. Data are presented as means ± SD (n ≥ 10, technical replicate). (B) The Papp values of lucifer yellow in the WT iPSC-IEC and PEPT1-KO iPSC-IEC monolayers were measured. Data are presented as means ± SD (n ≥ 3, technical replicate). (C) The apical-to-basolateral permeability of glycylsarcosine across the WT iPSC-IEC and PEPT1-KO iPSC-IEC monolayers was measured. Data are presented as means ± SD (n = 3, technical replicate). (D) The glycylsarcosine uptake capacity in the WT iPSC-IECs and PEPT1-KO iPSC-IECs was examined. Data are presented as means ± SD (n ≥ 3, technical replicate). Statistical analyses were performed using the unpaired two-tailed Student’s t test (*p < 0.05, ***p < 0.001).