. 2019 Jul 30;26:69–72. doi: 10.1016/j.mmcr.2019.07.006

Table 1.

Polymerase chain reaction (PCR) protocol using sequencing of international transcribed spacer (ITS) region.

Step	Temp.(°C)	Time (sec.)	Round
Pre-denature	94	60	1
Denature	94	30	35
Annealing	48	30
Extension	68	60
Final extension	68	300	1
Hold at	4	α