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CRISPR/Cas genome editing in C. thermocellum. A. C. thermocellum breaks down recalcitrant lignocellulosic biomass to produce biofuels. The robust lignocellulose activity of C. thermocellum make them a top candidate for consolidated bioprocessing. B. Schematic of the C. thermocellum native Type I–B operon (Clo1313_2969–2976) and heterologous Type II Cas9 CRISPR/Cas systems.
