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Toxicity Assay. A. A toxicity assay wherein the RNP targets the transformed plasmid. If the RNP is active, then cleavage of the plasmid will occur, destroying the plasmid conferring resistance to the selection. B. A toxicity assay wherein the RNP targets the chromosome. If the RNP is active, then cleavage of the chromosome will occur, resulting in cell death. C. Table describing the results of the toxicity assay for various systems tested.
