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. 2019 Dec 19;13(2):125–134. doi: 10.1016/j.tranon.2019.10.019

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

FZD1 connects BMP4-Smad1 signaling with Wnt pathway. (A) Top 20 highly activated pathways were listed in comparison of BMP4 vs DMSO group and BMP4 vs BMP4+LDN group from RNA-sequencing results. (B) The mRNA expression of FZD1 was continuously increased under the treatment of BMP4 in a time-dependent manner. (C) Western blot showed that BMP4 treatment increased the expression of Id1 and FZD1. The expression of FZD1 started to increase at day 3. (D) TS603 cells were treated with BMP4 for 72 h. ChIP-qPCR revealed that BMP4-Smad1 signaling directly regulated the expression of FZD1. (E–F) BMP4 and CM treatment of TS603 for 24 h significantly upregulated the expression of FZD1. Both of LDN-193189 and BMP4 neutralizing antibody could block the effect of BMP4-induced FZD1 expression. **P < 0.01; ***P < 0.001.