Figure 4.

Upregulation miR-31-5p Hinders HCC Progression and Chemoresistance of HepG2 Cells to Cisplatin by Depleting MAGEA3

(A) The binding sites of miR-31-5p and miR-448 in the 3′ UTR region of MAGEA3 predicted by TargetScan. (B) The luciferase activity of MAGEA3-WT and MAGEA3-Mut in HepG2 cells after miR-31-5p or miR-448 mimic transfection. (C–E) The viability (C), invasion (×200; D), and cisplatin-induced apoptosis (E) of HepG2 cells following miR-31-5p mimic transfection evaluated by MTT assay, Transwell assay, and flow cytometry, respectively. (F) IC₅₀ value of HepG2 cells following miR-31-5p mimic transfection. (G) Western blot analysis showing protein expression of MRP2, MRP3, MDR-1, and E-cadherin in HepG2 cells after restoration of miR-31-5p. (H) Content of ALB in supernatant of HepG2 cells following overexpression of miR-31-5p detected by ELISA. Measurement data were expressed as mean ± SD. The comparison between the two groups was analyzed by independent sample t test and the comparisons among multiple groups by one-way ANOVA, followed by Tukey’s post hoc test. Each experiment was repeated three times. *p < 0.05 versus the NC-transfected cells.