Fig. 9.

Effect of GSH depletion on the activity of the Trx system in J774A.1 cells. Cells (1 × 10⁵ cells well⁻¹) were treated with 25 μM BSO for 24 h, and then incubated with different concentrations of BQ (10–80 μM) for another 1 h. Trx activity was measured using the insulin reduction assay (A), TrxR activity was measured by the DTNB assay (B). *p < 0.05, **p < 0.01 vs. control (CON) samples with no BSO pretreatment. The relative activities for all groups are referenced to the control group without BSO and BQ treatment (indicated as CON; 0 μM, black bars), with this value taken as 100%.