FIGURE 1.

Flow chart showing the number of subjects recruited, time points for blood sampling, number of PBMC samples analyzed by flow cytometry, and the Ab panels used for the experiments. A total of 134 ELBW preterm infants and 29 FT control subjects were recruited for the study. PBMCs were isolated from 76 ELBW infants from D14, 50 ELBW infants from D28, and 59 ELBW infants from PMW 36 + 0 wk. PBMCs from 28 infants were available from all three time points, and PBMCs from 48 neonates were available from at least two time points. FACS panels were designed to characterize conventional T cells, their corresponding subsets, and the homing markers. The number of PBMC samples analyzed by FACS varied in different panels depending on the adequacy of the cells.