FIGURE 4.

ELBW preterm infants have a reduced T-bet/GATA3 ratio in CD4⁺ T cells at 14 and 28 d of age. The expression levels of (A) GATA3, (B) T-bet, and (C) RORγt in the CD4⁺ T cells of control FT neonates at D14 and the ELBW preterm infants at D14, D28, and at PMW 36 + 0. The presented data of geometric mean fluorescence intensity (geo MFI) for transcription factors analyses was done following gating on CD4 cells that are positive for these factors. (D) Ratio between the percentages of T-bet– and GATA3-expressing CD4⁺ T cells. Bars show medians with interquartile range. Kruskal-Wallis test with Dunn multiple comparison tests was used to make group comparisons.