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Loss of CD53 alters downstream gene expression. Gene expression of canonical PI3K target genes. RNA was isolated from sorted B cell populations and qRT-PCR was performed for Pax5, Il-7r, BIM, Ccnd1, and Rag2, relative to Actb (n = 11 mice per group, age 5 wk) over three independent experiments. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01 by an unpaired Student t test.
