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(A) Replicates of the standard curve analysis show that the limit of detection of the M. musculinus PCR assay is 100 copies. (B) Detection of M. musculinus in swab samples by using MC28S qPCR assay for groups 1 (infested with M. musculinus and D. musculi), 2 (M. musculi and R. affinis), and 3 (M. musculinus and M. musculi). (C). Detection of M. musculinus in fecal samples by using the MC28S qPCR assay for groups 1, 2, and 3.
