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(i) In solid‐phase transfection, the microwell plates are coated with the transfection mixes consisting of synthetic gRNAs, lipid reagent, sucrose, and gelatin. The microwell plates are then freeze dried and can either be stored for long periods of time or (ii) the cells can directly be seeded on these pre‐coated plates. A wide range of readouts such as microscopy, flow cytometry, or cell viability assays is possible.
