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. 2019 Nov 13;294(51):19804–19813. doi: 10.1074/jbc.RA119.009435

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© 2019 Liu et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — MOV10 impairs HBV reverse transcription. A, HepG2 cells were co-transfected with the wide type, or RH-defective HBV construct (0.5 μg) along with the MOV10-expressing plasmid (1 μg), or control vector. A and B, three days after transfection, extracted intracellular HBV DNA was analyzed by Southern blotting (A) and qPCR (B). The positions of RC, double-strand linear (DSL), and ssDNA intermediates are indicated. C, schematic presentation of HBV pgRNA and (−)DNA that was synthesized by RH-defective Pol. Positions of the sequences amplified by qPCR are indicated with numbered black lines. Intracellular (−) DNA was analyzed by qPCR with different sets of primers. Results from three separate experiments are shown as mean values ± S.D. *, p < 0.05 (Student's t test).