Figure 5.

Oleate is a critical regulator of FGF21 expression. 12-week-old LOX and LKO mice were fed either an HCD, triolein, or tristearin for 10 days. Mice were euthanized after 4 h of fasting, and plasma samples were collected. A and B, relative FGF21 expression and plasma protein levels (A) and blood glucose levels (B) after 4 h of fasting. Values are mean ± S.E. (n = 3–5/group). *, p < 0.05 versus LOX counterparts by Student's two-tailed t test. C, FGF21 expression in primary hepatocytes treated with an SCD1 inhibitor (SCD Inh) or SCD1 inhibitor in combination with palmitate (16:0), palmitoleate (16:1), or oleate (18:1).