Figure 6.
Inhibition of LasA and LasB by their propeptides. A, active LasA and LasB were mixed with purified LasApp and LasBpp at various molar ratios. After 30-min incubation, the activities of LasA and LasB were measured. B and C, mature forms of LasB (100 ng), PIV (100 ng), and LasA (500 ng) were mixed with 1 μg of LasApp (B) or 1 μg of LasBpp (C), incubated for 30 min, and electrophoresed in 15% SDS-PAGE. D, P-LasA (mature LasA, 500 ng) was mixed with LasApp (850 ng), and mature LasB (100 ng) or PIV (100 ng) was then added to the mixture. LasA activity was measured after 30-min incubation. ***, p < 0.005