Figure 10.

ER stress does not alter Deg1-Sec62 membrane association. ER-derived microsomes were prepared from hrd1Δ yeast expressing Deg1-Sec62 that had been cultured in the presence or absence of 6 mm DTT for 1 h. Microsomal fractions were incubated in the presence of water, 1% Triton X-100, 0.5 m sodium carbonate, or 0.5 m sodium chloride before being separated into pellet (P) and supernatant (S) fractions and solubilized. Deg1-Sec62 was detected with peroxidase anti-peroxidase antibodies. Cue1 and Cdc48 were detected by anti-Cue1 and anti-Cdc48 antibodies, respectively. The experiment depicted was performed two times (with the exception of sodium chloride treatment, which was performed one time).