Skip to main content
. 2019 Nov 13;294(51):19814–19830. doi: 10.1074/jbc.RA119.010295

Figure 5.

Figure 5.

ER stress differentially affects degradation of Asi INMAD substrates. A, schematic of Asi substrates investigated in this figure. B, cycloheximide chase analysis of yeast of the indicated genotypes expressing Erg11-FLAG or Deg1-Sec62 cultured in the presence of 6 mm DTT, 10 μg/ml tunicamycin, or DMSO for 1 h. C, cycloheximide chase analysis of yeast of the indicated genotypes expressing Vtc1-3HA and Deg1-Sec62 cultured in the absence or presence of 6 mm DTT for 1 h. DTT, tunicamycin, and DMSO were maintained at the same concentration during incubation with cycloheximide. Erg11-FLAG was detected with anti-FLAG antibodies. Vtc1-3HA was detected with anti-HA antibodies. Deg1-Sec62 was detected with peroxidase anti-peroxidase antibodies. Pgk1 served as a loading control. The experiment depicted in B was performed two times. The experiment depicted in C was performed three times (with the exception of tunicamycin treatment, which was performed one time).