Figure 7.

Deg1-Sec62 induces the unfolded protein response. Abundance of the UPR reporter GFP (driven by the UPRE) was analyzed by flow cytometry in WT and ubc7Δ yeast. Mid-exponential–phase cells harboring an empty vector or a plasmid encoding Deg1-Sec62 under the control of the GPD promoter were incubated in the absence (vector and Deg1-Sec62) or presence (vector) of 6 mm DTT for 1 h. The mean fluorescence intensity of 10,000 cells from each of nine cultures for each condition was normalized to the average mean fluorescence intensity of nine cultures of untreated WT cells harboring a vector. Mean fluorescence intensity ± standard error of the mean is presented. A two-tailed unpaired t test was performed to determine the significance of the difference between ubc7Δ cells harboring an empty vector and those expressing Deg1-Sec62.