Table 2.
Plasmids used in this study
| Name | Alias | Yeast selection marker | Yeast plasmid type | Description | Source | Figs. |
|---|---|---|---|---|---|---|
| pHA-Pdr5* | pVJ1/pRH2312 | HIS3 | CEN | HA-tagged Pdr5*; Pdr5* = C1427Y | 24 | 2E |
| YCp50-PPRC1-CPY*-HA | pVJ2/pDN431 | URA3 | CEN | HA-tagged CPY* driven by native promoter; CPY* = G255R | 92 | 2B |
| pRS313 | pVJ26 | HIS3 | CEN | Empty vector | 93 | 4C and 7 |
| pRS316 | pVJ27 | URA3 | CEN | Empty vector | 93 | 2, B and C, 3C, 4C, 8, A and C, and 11C |
| p416-PMET25-Deg1-FLAG-Sec62-2×ProtA | pVJ29 | URA3 | CEN | Deg1-Sec62 driven by MET25 promoter | 25 | 1, D, E, G, and H, 4C, 5, B and C, 10, and 11B, and S1 |
| p414-PMET25-Deg1-FLAG-Sec62-2×ProtA | pVJ30 | TRP1 | CEN | Deg1-Sec62 driven by MET25 promoter | 94 | 1, C and F, 2, D and E, 3, C and D, and 9, A and B |
| pRS314 | pVJ39 | TRP1 | CEN | Empty vector | 93 | 3C |
| pRS425 | pVJ43 | LEU2 | 2μ | Empty vector | 95 | 9B |
| p415-PMET25 | pVJ122 | LEU2 | CEN | Empty vector with MET25 promoter | 76 | 8D and 11A |
| p414- PMET25-Deg1-FLAG-Vma12-2×ProtA | pVJ172 | TRP1 | CEN | Deg1-Vma12 driven by MET25 promoter | 21 | 3B |
| p414-PMET25-Deg1*-FLAG-Vma12-2×ProtA | pVJ177 | TRP1 | CEN | Deg1*-Vma12 driven by MET25 promoter; Deg1* = F18S, I22T | 21 | 3B |
| p414-PMET25-Deg1-FLAG-sec62†-2×ProtA | pVJ204 | TRP1 | CEN | Deg1-sec62† driven by MET25 promoter; sec62† = sec62-1 = G127D | 25 | 3D |
| p416-PMET25-Deg1*-Sec62-2×ProtA | pVJ317 | URA3 | CEN | Deg1*-Sec62 driven by MET25 promoter; Deg1* = F18S, I22T | 25 | 8, A and C, and 11C |
| p416-PGPD-Deg1-FLAG-Vma12-2×ProtA | pVJ343 | TRP1 | CEN | Deg1-Vma12 driven by TDH3 (GPD) promoter | This study | 3C |
| p415-P PMET25-Deg1*-Sec62-2×ProtA | pVJ411 | LEU2 | CEN | Deg1*-Sec62 driven by MET25 promoter; Deg1* = F18S, I22T | This study | 8D and 11A |
| p413-PGPD-FLAG-Vma12-ProtA-K12-13myc | pVJ457 (STK 07.4.3) | HIS3 | CEN | Vma12-K12-13myc driven by TDH3 (GPD) promoter | 8 | 4C |
| p413-PGPD-Deg1-Sec62-2×ProtA | pVJ463 | HIS3 | CEN | Deg1-Sec62 driven by TDH3 (GPD) promoter | Gift of S. Kreft | 7 |
| pMRS366 | pVJ512 | URA3 | CEN | Empty vector | 96 | 9A |
| pMR397 (CEN) | pVJ513 | URA3 | CEN | Kar2 expression plasmid | 96 | 9A |
| YGPM17a24 | pVJ532 | LEU2 | 2μ | Plasmid from Yeast Genomic Tiling Collection with genomic segment, including KAR2 gene and promoter | 63 | 9B |
| p416-PERG3-ERG3-13Myc | pVJ533/pLJ001 | URA3 | CEN | 13myc-tagged Erg3 driven by native promoter | 51 | 2C |
| pRS314-UPRE-GFP | pVJ552 | TRP1 | CEN | GFP driven by unfolded Protein Response Element (UPRE) | 97 | 7 and 11A |
| pTDH3-SUS-GFP | pRH2900 | TRP1 | YIp | SUS-GFP driven by TDH3 (GPD) promoter | 54 | Used to generate MHY10483 |
| pRS316-Erg11-FLAG | URA3 | CEN | FLAG-tagged Erg11 driven by native promoter | This study | 5B |