Figure 5.

Focus-Revert-Classify Strategy Results in at Least Six Different CI Open Structures

(A) Schematic of focus-revert-classify strategy for separating CI particles on the basis of the angle between the peripheral and membrane arms.

(B) Overlay of CI models generated from the focus-revert-classify strategy aligned by the CI membrane arm. Models colored with the closed state blue and the different open states from dark green to yellow-green according to overall resolution. Viewed from CI side (left) and the mitochondrial matrix (right). Differences in the relative positions of the CI peripheral arm indicated by arrows.

(C) Slice through the CI peripheral arm at the position indicated by red dashed line in (B). Models shown as ribbons and colored as in (B). Approximate boundaries between subunits are indicated by black lines, subunits labeled.

See also Figure S6.