Figure 4.

In Vivo Structure-Function Analysis of GluD1 Function at Inhibitory Synapses

(A) EGFP-gephyrin clusters in representative segments of oblique dendrites in control condition (shControl) or after in utero replacement of endogenous GluD1 with indicated mutants in P20–22 mice. Dashed lines, dendritic contours based on tdTomato fluorescence. Scale bar: 2 μm.

(B) Schematic of a GuD1 subunit and localization of the indicated mutations. GluD1 receptors interact with Cbln bound to presynaptic neurexin via their NTD and use glycine or D-serine as agonists. NTD, N-terminal domain; ABD, agonist-binding domain; TMD, transmembrane domain; CTD, C-terminal domain.

(C) Quantification of GPHN cluster density in conditions represented in (A).

Histogram represents means ± SEM. Data corresponding to shControl, shGluD1, and shGluD1 + GluD1^∗ are the same as in Figure 1G. n_shControl = 41, n_shGluD1 = 30, n_{shGluD1 + GuD1^∗} = 32, n_{shGluD1 + GluD1 ΔNTD} = 30, n_{shGluD1 + GluD1 R3341A/W343A} = 32, n_{shGluD1 + GluD1 R526K} = 30, n_{shGluD1 + GluD1 V617R} = 29, n_{shGluD1 + GluD1 ΔCTD} = 28. ns p > 0.05, ^∗∗∗p < 0.001, determined by one-way ANOVA test followed by Tukey’s post test.