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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: J Am Chem Soc. 2019 Dec 9;141(50):19625–19633. doi: 10.1021/jacs.9b07111

Figure 3 ∣. 31P NMR analysis of a single turnover TglB reaction using 18O labeled TglA.

Figure 3 ∣

The 18O incorporation in the product phosphate strongly supports mechanism II in Figure 2C.