. Author manuscript; available in PMC: 2020 Dec 18.

Published in final edited form as: J Am Chem Soc. 2019 Dec 9;141(50):19625–19633. doi: 10.1021/jacs.9b07111

Table 1 ∣. Competition binding of TglA-Ala variants to TglB.

A fluorescein labeled peptide corresponding to the C-terminal 20 amino acids of TglA was competed off with full length TglA and its mutants. FP traces are shown in Figure S1.

TglA variant	IC₅₀ (μM)	TglA variant	IC₅₀ (μM)
WT TglA	8 ± 1	E45A	> 300
D40A	15 ± 3	S46A	11 ± 2
I41A	32 ± 4	K47A	255 ± 63
E42A	10.5 ± 0.3	V48A	113 ± 36
V43A	98 ± 36	F49A	4.2 ± 0.2
I44A	24 ± 2