Fig. 6.

Experimental validation of RADAR-detected DM sites using the SELECT method. We applied antibody independent method SELECT on T2D samples (N = 4). Shown are SELECT results of six putative DM sites for validation. SELECT measures the relative abundance of non-methylated RNA molecules of target locus as represented by the elongation and ligation “read through” of oligo probes. Thus, SELECT results—“relative read through”—are inversely correlated with m⁶A level