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. Author manuscript; available in PMC: 2020 Mar 29.
Published in final edited form as: Science. 2019 Mar 28;363(6434):1463–1467. doi: 10.1126/science.aaw1219

Figure 1: High-resolution RNA capture from tissue by Slide-seq.

Figure 1:

(A) Left: Schematic of array generation. A monolayer of randomly deposited, DNA barcoded beads (termed a “puck”) is spatially indexed by SOLiD sequencing. Top Right: A representative puck with sequenced barcodes shown in black. Bottom Right: A composite image of the same puck colored by the base calls for a single base of SOLiD sequencing. (B) Schematic of the sample preparation procedure developed for Slide-seq. (C) Top left: tSNE representation of Slide-seq beads from a coronal mouse hippocampus slice with colors indicating clusters. Right: the spatial position of each bead is shown, colored by the cluster assignments shown in the tSNE. Bottom left: Inset indicating the position of a single-cell-thickness ependymal cell layer (black arrow). (D) As in (C), but for the indicated tissue type (see Fig. S2 for clustering and cluster identities). All scale bars 500 μm.