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. 2019 Aug 13;11(11):967–978. doi: 10.1093/jmcb/mjz084

Figure 4.

Figure 4

Reconstitution of the expression of detyr-α-tub attenuates the fusion deficiency caused by TTL overexpression. (A) TTL in BeWoTTL (TTL-overexpressing) cells was knocked down using siRNA. Western blotting was performed using anti-detyr-α-tub, anti-TTL, and anti-GAPDH antibodies. The fusion index was determined contemporaneously with the samples harvested for western blotting. **P < 0.01, *P < 0.05, n = 3. (B) Western blotting of BeWoVASH2 cells (with BeWoGFP serving as a control line) treated with DMSO (control) or forskolin for 48 h using anti-detyr-α-tub, anti-TTL, anti-Flag, or anti-GAPDH antibodies. The fusion index was determined contemporaneously with the samples harvested for western blotting. **P < 0.01, *P < 0.05, n = 3. (C) Western blotting of BeWoTTLmut cells (with BeWoGFP and BeWoTTL cells serving as controls) treated with DMSO or forskolin for 48 h. Western blotting was performed using anti-detyr-α-tub, anti-TTL, anti-Flag, or anti-GAPDH antibodies. The fusion index was determined contemporaneously with the samples harvested for western blotting. **P < 0.01, *P < 0.05, n = 3.