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. 2019 Feb 12;11(11):979–993. doi: 10.1093/jmcb/mjz008

Figure 3.

Figure 3

Overexpression of ANO6 enhances ADAM10 sheddase activity upon calcium influx. (A) Mock-transfected and Anoctamin-6 (ANO6)-GFP-transfected COS7 cells were stimulated with ionomycin (IO, 1 μM) for the indicated time. After stimulation, cells were stained with Annexin V-APC and analysed via FACS analysis. (B and C) COS7 cells were co-transfected with ANO6-GFP or mock vector and the AP-tagged ADAM10 substrates BTC or VE-cadherin, respectively. Cells were stimulated with IO (1 μM) for 30 min. IO-induced shedding was significantly increased upon overexpression of ANO6. TAPI (10 μM), ADAM10 inhibitor GI (3 μM), and OPS (10 mM), but not OPC (10 mM), significantly abrogated the induced shedding. * indicates a significant increase compared to mock-transfected stimulated cells; # indicates significant decrease compared to ANO6-transfected stimulated control cells (P < 0.05, n = 3; ±SEM). NS, no significant difference. Data were analysed by one-way analysis of variance and Bonferroni multiple comparison post hoc test. CO, control.